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Akt activation before and after insulin stimulation. A, Akt phosphorylation was quantified in murine primary myotubes after serum starvation for 20 h and treatment with vehicle or 1 or 10 nm insulin for 5 min. A representative blot is shown. B, Pooled densitometry results for three experiments. Bars, mean ± sem. *, P < 0.05 vs. wild type (WT).
