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. 2008 Dec 18;150(4):1588–1596. doi: 10.1210/en.2008-1001

Figure 4.

Figure 4

ERα transcriptional activity and expression in MCF-7 cells after depletion of SRC coactivators. A, MCF-7 cells were transfected with 10 pmol total siRNA specific for Ambion's negative control siRNA no. 2 (siCont), SRC-1 (siSRC-1), SRC-2 (siSRC-2), or SRC-3 (siSRC-3). Twenty-four hours later, cells were transfected with 1 μg ERE-E1b-LUC reporter plasmid, and 1 d later cells were exposed to VEH (0.1% ethanol) or 1 nm E2 for an additional 24 h. Luciferase activity was assessed and normalized to total protein. Data are presented as ER activity relative to values obtained for E2-treated, control siRNA-transfected samples and represent the mean ± sem of six experiments. Statistical analyses were by one-way ANOVA: *, P < 0.05; or **, P < 0.01 vs. similarly treated (VEH or E2) control siRNA-transfected cells. Parallel MCF-7 cells (B) treated with either vehicle (V) or E2 were assessed for ERα and actin expression by Western blot analysis. A representative Western blot is shown.