Skip to main content
. 2008 Dec 18;150(4):1588–1596. doi: 10.1210/en.2008-1001

Figure 5.

Figure 5

Effect of p160 coactivator depletion on mRNA expression of ERα target genes. MCF-7 cells were transfected with siRNA targeting luciferase-negative control (siCont), SRC-1 (siSRC-1), SRC-2 (siSRC-2), or SRC-3 (siSRC-3) and 24 h later were treated with VEH (0.1% ethanol), 1 nm E2, or 100 nm 4HT for 90 min (c-myc; A), 3 h (cyclin D1, E), or 24 h (pS2, PR, and Bcl-2; B–D, respectively). Cells were then harvested for RNA isolation and measurement of the indicated mRNAs by RT-qPCR. The data are normalized to E2-treated control siRNA samples and represent the mean ± sem of three to four experiments. Statistical analyses were by one-way ANOVA: *, P < 0.05; or **, P < 0.01 vs. similarly treated (VEH, E2, or 4HT) control siRNA-transfected cells. Levels of SRC-1 (F), SRC-2 (G), and SRC-3 (H) mRNAs are shown for MCF-7 cells transfected with control or p160 coactivator siRNAs and treated with VEH for 24 h. Values represent mean ± sem of three experiments.