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. 2008 Dec 18;150(4):1588–1596. doi: 10.1210/en.2008-1001

Figure 6.

Figure 6

Effect of p160 coactivator depletion on protein expression of ERα target genes. MCF-7 cells transfected with control (C), SRC-1 (S1), SRC-2 (S2), or SRC-3 (S3) siRNAs were treated with VEH or E2 for 90 min (c-myc) or 24 h (PR and Bcl-2), harvested and whole-cell lysates assessed for c-myc (A), PR (B), and Bcl-2 protein (C) levels by Western blotting. Blots obtained from three to four independent experiments were quantitated by densitometry and expressed as mean ± sem. Statistical analyses by t test: *, P < 0.05; or **, P < 0.001 vs. similarly treated (VEH or E2) control siRNA-transfected cells. Western blot analysis of constitutive Bcl-2 protein expression for untreated MCF-7 and LCC2 cells (D) or MCF-7 and LCC2 cells treated with siRNAs and exposed to E2 (E) were as described for C. Blots are representative of three separate experiments.