Skip to main content
. 2008 Nov 20;150(4):1739–1747. doi: 10.1210/en.2008-1202

Figure 2.

Figure 2

HPT axis regulation is altered in PAM+/− mice. Pituitary levels of TSH-β (A) and PAM (B) mRNA (normalized to GAPDH) were determined in euthyroid and hypothyroid animals by quantitative PCR. PAM+/− (n/group = 8–9) and WT (n/group = 8 each) mice were kept on the low-iodine/PTU diet or a normal diet for 14 d; error bars, sem. Two independent sets of animals were examined; data shown are a combination of both experiments (total = 42; n/group = 7–9). A two-way ANOVA revealed a significant effect of low-iodine/PTU diet on the levels of TSH (P < 0.0005) and a significant interaction (i.e. a difference in the effect of the diet) between genotype and the low-iodine/PTU diet (P < 0.0005; A). WT mice showed a more substantial increase in TSH mRNA to hypothyroidism than PAM+/− mice. The pituitary levels of PAM mRNA increased in response to hypothyroidism in WT mice but not PAM+/− mice. A second two-way ANOVA revealed a significant effect of low-iodine/PTU diet on the levels of PAM (P = 0.023) and a significant interaction between genotype and the low-iodine/PTU diet (P = 0.05; B). Asterisk, See Statistical analysis for P-values.