Temporal effects of eCG on BMPRIB, BMPRII, BMPRIA, and actin mRNA levels (A and B), and on BMPR protein expression (C). Six-day-old female hamsters were injected sc with 20 IU eCG or vehicle, and ovaries were collected at 0, 6, 12, and 24 h after the injection. P7 ovaries served as controls for the 24-h-exposure group. Immunofluorescence signal was quantified using the NIH Image J software, and the results were expressed as the mean OD/pixel ± sem. Bars with a different letter, P < 0.05; bars with the same letter, P > 0.05.