Figure 2.

Acute insulin responsiveness is impaired similarly by hyperinsulinemia and GlcN. Cells were treated exactly as described in Fig. 1. After treatments, cells were washed and either left untreated (basal) or acutely (30 min) challenged with 100 nm insulin (30′ Ins), and GLUT4 translocation and glucose transport were determined. A, Representative images of PM sheets subjected to immunofluorescence (IF) microscopy with GLUT4 antibody (panels 1–4 and 9–12) and WGA (panels 2–8 and 13–16); B, signal quantitation (means ± se) from three to five experiments. All microscopic and camera settings were identical between groups. C, 2-DG uptake (means ± se) from three to six independent experiments. *, P < 0.05 vs. control; #, P < 0.05 vs. control 30′ Ins. ns, Not significant.