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Population-based LI-COR Odyssey analyses quantitate a loss in PM PIP2 detection and cortical F-actin. For these analyses, PM sheets or cells on an entire 35-mm cell culture well were labeled as in Fig. 3, and the fluorescent signals in the entire well were quantitated using the LI-COR Odyssey system as described in Materials and Methods. PM PIP2 (A) and F-actin (B) means ± se from three independent experiments are shown. *, P < 0.05 vs. control; #, P < 0.05 vs. 12 h Ins.
