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PIP2 add-back restores GlcN-induced PIP2 decrease. A, During the final 60 min of control or GlcN incubation, the medium was replaced with the same medium enriched with either histone H1 (panels 1, 2, 4, and 5) or PIP2/histone H1 (panels 3 and 6), and representative immunofluorescent images of PM PIP2 (panels 1–3) and WGA-stained PM (panels 4–6); B, quantitation of PM PIP2. Images and means ± se are from three to four independent experiments. *, P < 0.05 vs. control.
