Figure 7.

PIP₂ replenishment protects against GlcN-induced F-actin loss and insulin resistance. Control and GlcN cells were treated with either histone H1 or PIP₂/histone H1 as described in Fig. 6. A, Phalloidin-stained F-actin and propidium iodide-stained nuclei in these cells; B, insulin-stimulated PM GLUT4 images; C, GLUT4 quantitation. All microscope and camera settings were identical between groups, and representative images from three independent experiments are shown. Values are means ± se from three to five independent experiments. *, P < 0.05 vs. control; #, P < 0.008 vs. 30′ Ins.