Figure 2.

Nascent RNA analysis of GR-induced genes in 3134. To evaluate more closely the transcriptional component of the complex regulatory profile, nascent transcripts were characterized for representative genes from each induction class. Nascent RNA levels were measured at multiple time points after induction by q-PCR analysis, using primer pairs spanning intron/exon boundaries for each gene. A, Strategy for nascent transcript mapping. Nascent transcript levels were determined after hormone addition for members of the transiently induced class [Tgm2 (B), Ampd3 (C), Mt2 (D), and Suox (E)], the rapid induction class [Sgk (F), Lcn2 (G), and Glul (H)], and the continuously induced class [Ednl (I)]. Error bars show the sd from the mean. q-PCR experiments represent three independent experiments (biological replicates) performed in duplicate (technical replicates).