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. 2008 Nov 20;150(4):1870–1878. doi: 10.1210/en.2008-0779

Figure 7.

Figure 7

Effect of ovarian steroids (administered as a regime that induces a preovulatory surge of gonadotropins) on MBH and POA Eap1 mRNA levels measured by real-time PCR in 31-d-old OVX rats. Eap1 mRNA levels in the POA or CTX were unaffected by the steroid treatments. EB alone (100 μg/kg) caused a small (*, P < 0.05; ANOVA followed by Student-Newman-Keuls test) decrease in Eap1 mRNA levels in the MBH at 24 h but had no discernible effect at the time of the EB-induced preovulatory surge of LH 54 h after injection. P (1 mg/rat) administered to EB-treated rats 6 h before killing resulted in a small increase in Eap1 mRNA content in the MBH (*, P < 0.05) but not the POA. Numbers in parentheses on top of bars are number of animals per group. Vertical lines are sem. Inset, Changes in serum LH levels 12, 24, and 54 h after a single dose of EB or 6 h after injecting EB-treated rats with a single dose of P. Values beyond the upper limit of sensitivity of the assay were assigned an arbitrary value of 500 ng/ml.