Table 1.
Effects of FSH treatment on testicular transcript levels – highest-regulated transcripts from microarray studiesa
| Gene symbol | Gene title | Fold change | Gene symbol | Gene title | |
|---|---|---|---|---|---|
| Fold change | |||||
| Transcripts up-regulated 12 h after start of treatment | Transcripts down-regulated 12 h after start of treatment | ||||
| 20·7 | Ren1 | Renin 1 structural | 7·27 | Myh8 | Myosin, heavy polypeptide 8 |
| 18·2 | Dmkn | Dermokine | 6·65 | Rin2 | Ras and Rab interactor 2 |
| 13·3 | Aqp5 | Aquaporin 5 | 5·03 | Pdgfc | Platelet-derived growth factor C |
| 12·1 | Wif1 | Wnt inhibitory factor 1 | 4·55 | Transcribed locusb | |
| 11·0 | Fabp5 | Fatty acid binding protein 5, epidermal | 4·53 | Bcan | Brevican |
| 6·6 | Tubb3 | Tubulin, β 3 | 4·46 | Rgs11 | Regulator of G-protein signalling 11 |
| 6·5 | Cyp17a1 | Cytochrome P450, family 17a 1 | 4·45 | Tmem37 | Transmembrane protein 37 |
| 6·3 | Akr1b7 | Aldo-keto reductase family 1 B7 | 4·43 | Fhod3 | Formin homology 2 domain containing 3 |
| 6·0 | Lgals1 | Lectin, galactose binding, soluble 1 | 4·38 | Derl3 | Der1-like domain family, member 3 |
| 5·9 | Col4a1 | Procollagen, type IV, α1 | 4·01 | Ddit4l | DNA-damage-inducible transcript 4-like |
| 5·7 | Pappa | Pregnancy-associated plasma protein A | 3·98 | BC013672 | cDNA sequence BC013672 |
| 5·2 | Star | Steroidogenic acute regulatory protein | 3·97 | Scin | Scinderin |
| 4·6 | Ldlr | Low density lipoprotein receptor | 3·96 | Ddit4l | DNA-damage-inducible transcript 4-like |
| 4·6 | Tesc | Tescalcin | 3·89 | Cabc1 | Chaperone, ABC1 complex-like |
| 4·6 | Rps6ka2 | Ribosomal protein S6 kinase 2 | 3·86 | Krt20 | Keratin 20 |
| 4·6 | Scara5 | Scavenger receptor class A 5 | 3·74 | Tmem140 | Transmembrane protein 140 |
| 4·4 | Hgsnat | Heparan N-acetyltransferase | 3·71 | Dbp | D site albumin promoter binding protein |
| 4·4 | Hs3st1 | Heparan sulphate 3-O-sulphotransferase 1 | 3·71 | Rnasel | Ribonuclease L |
| 4·2 | Syne1 | Synaptic nuclear envelope 1 | 3·70 | Spsb1 | splA receptor domain and SOCS box 1 |
| 4·1 | Slc38a5 | Solute carrier family 38, member 5 | 3·67 | Tnni3 | Troponin I, cardiac |
| 4·0 | Gpd1 | Glycerol-3-phosphate dehydrogenase 1 | 3·65 | Cdo1 | Cysteine dioxygenase 1, cytosolic |
| 3·9 | Dos | Downstream of Stk11 | 3·57 | Stard8 | START domain containing 8 |
| 3·8 | Svs5 | Seminal vesicle secretory protein 5 | 3·55 | Slc40a1 | Solute carrier family 40, member 1 |
| 3·8 | Bhmt | Betaine-homocysteine methyltransferase | 3·52 | Hdac5 | Histone deacetylase 5 |
| 3·7 | D9Ertd280e | Chr 9, ERATO Doi 280 | 3·32 | Dbp | D site albumin promoter binding protein |
| 3·7 | Tnfrsf12a | Tumour necrosis factor receptor 12a | 3·23 | Chdh | Choline dehydrogenase |
| 3·7 | 1200016E24Rik | RIKEN cDNA 1200016E24 | 3·23 | 8030411F24Rik | RIKEN cDNA 8030411F24 gene |
| 3·7 | Nr4a1 | Nuclear receptor subfamily 4, group A1 | 3·23 | Per3 | Period homolog 3 (Drosophila) |
| 3·6 | Dkk3 | Dickkopf homolog 3 | 3·22 | Ctnna2 | Catenin, α 2 |
| 3·6 | Cyp51 | Cytochrome P450, family 51 | 3·19 | Trim47 | Tripartite motif protein 47 |
| Transcripts up-regulated 24 h after start of treatment | Transcripts down-regulated 24 h after start of treatment | ||||
| 28·8 | Lin7c | Lin-7 homolog C (C. elegans) | 9·9 | Ddit4l | DNA-damage-inducible transcript 4-like |
| 18·0 | Cyp17a1 | Cytochrome P450, family 17a1 | 9·9 | Rin2 | Ras and Rab interactor 2 |
| 13·6 | Cyp11a1 | Cytochrome P450, family 11a1 | 9·1 | Slc40a1 | Solute carrier family 40, member 1 |
| 11·0 | Fabp5 | Fatty acid binding protein 5, epidermal | 8·0 | Rgs11 | Regulator of G-protein signalling 11 |
| 10·1 | Rhox5 | Reproductive homeobox 5 | 7·6 | Igfbp3 | Insulin-like growth factor binding protein 3 |
| 9·4 | Star | Steroidogenic acute regulatory protein | 7·4 | Myh6 | Myosin, heavy polypeptide 6 α |
| 8·7 | Slc38a5 | Solute carrier family 38, member 5 | 7·2 | Apbb2 | Amyloid precursor protein-binding B2 |
| 7·9 | Aqp5 | Aquaporin 5 | 6·7 | Rassf5 | Ras association domain family 5 |
| 7·4 | Tubb3 | Tubulin, β 3 | 6·3 | Tmem37 | Transmembrane protein 37 |
| 7·0 | Drd4 | Dopamine receptor 4 | 6·3 | Chdh | Choline dehydrogenase |
| 6·3 | Tesc | Tescalcin | 6·2 | Itga9 | Integrin α 9 |
| 5·5 | Lgals1 | Lectin, galactose binding, soluble 1 | 6·2 | Thbd | Thrombomodulin |
| 5·0 | Spinlw1 | Eppin | 5·9 | Fcgr2b | Fc receptor, IgG, low affinity IIb |
| 4·6 | Osr1 | Odd-skipped related 1 | 5·8 | Trim47 | Tripartite motif protein 47 |
| 4·5 | Fads2 | Fatty acid desaturase 2 | 5·7 | Spsb1 | splA receptor domain and SOCS box 1 |
| 4·2 | Pappa | Pregnancy-associated plasma protein A | 5·6 | Ddit4l | DNA-damage-inducible transcript 4-like |
| 4·2 | Scara5 | Scavenger receptor class A5 | 5·5 | Vnn1 | Vanin 1 |
| 4·2 | Pscdbp | Pleckstrin homology binding protein | 5·5 | Fcgr2b | Fc receptor, IgG, low affinity IIb |
| 4·1 | Plac8 | Placenta-specific 8 | 5·4 | Ptprd | Protein tyrosine phosphatase, receptor D |
| 4·0 | Gpt2 | Glutamic pyruvate transaminase 2 | 5·4 | Nkx3-1 | NK-3 transcription factor, locus 1 |
| 4·0 | Rps6ka2 | Ribosomal protein S6 kinase 2 | 5·3 | Fcgr2b | Fc receptor, IgG, low affinity IIb |
| 4·0 | Gpd1 | Glycerol-3-phosphate dehydrogenase 1 | 5·1 | Ctgf | Connective tissue growth factor |
| 4·0 | Hdc | Histidine decarboxylase | 5·0 | H19 | H19 fetal liver mRNA |
| 3·8 | Igf1 | Insulin-like growth factor 1 | 5·0 | Pdgfc | Platelet-derived growth factor C |
| 3·7 | Fah | Fumarylacetoacetate hydrolase | 4·9 | Hsd17b11 | Hydroxysteroid (17β) dehydrogenase 11 |
| 3·7 | Mpzl2 | Myelin protein zero-like 2 | 4·9 | Cabc1 | Chaperone, ABC1 complex-like |
| 3·6 | Insl3 | Insulin-like 3 | 4·9 | 9630031F12Rik | RIKEN cDNA 9630031F12 gene |
| 3·4 | Wif1 | Wnt inhibitory factor 1 | 4·8 | Ptch1 | Patched homolog 1 |
| 3·3 | Inha | Inhibin α | 4·7 | Smoc2 | SPARC related modular calcium binding 2 |
| 3·2 | Col18a1 | Procollagen, type XVIII, α 1 | 4·7 | Fhod3 | Formin homology 2 domain containing 3 |
| Transcripts up-regulated 72 h after start of treatment | Transcripts down-regulated 72 h after start of treatment | ||||
| 35·0 | Drd4 | Dopamine receptor 4 | 13·4 | Igfbp3 | Insulin-like growth factor binding protein 3 |
| 21·1 | Slc38a5 | Solute carrier family 38a5 | 8·2 | Rgs11 | Regulator of G-protein signalling 11 |
| 14·9 | Rhox5 | Reproductive homeobox 5 | 6·6 | Rin2 | Ras and Rab interactor 2 |
| 13·9 | Fabp5 | Fatty acid binding protein 5, epidermal | 6·5 | Clca1 | Chloride channel calcium activated 1 |
| 8·4 | Spinlw1 | Eppin | 6·0 | Slc40a1 | Solute carrier family 40 1 |
| 7·6 | Klk1b24 | Kallikrein 1-related peptidase b24 | 5·6 | Spsb1 | splA receptor domain and SOCS box 1 |
| 6·6 | Tubb3 | Tubulin, β 3 | 4·9 | Ifitm1 | Interferon induced transmembrane 1 |
| 5·9 | Hdc | Histidine decarboxylase | 4·7 | Myh8 | Myosin, heavy polypeptide 8 |
| 5·5 | Fah | Fumarylacetoacetate hydrolase | 4·6 | Fhod3 | Formin homology 2 domain containing 3 |
| 5·5 | Tsx | Testis specific X-linked gene | 4·4 | Bcan | Brevican |
| 5·4 | Zcchc18 | Zinc finger, CCHC domain 18 | 4·4 | Tmem140 | Transmembrane protein 140 |
| 4·6 | Sct | Secretin | 4·4 | BC013672 | cDNA sequence BC013672 |
| 4·6 | Gpd1 | Glycerol-3-phosphate dehydrogenase 1 | 4·3 | Tmem37 | Transmembrane protein 37 |
| 4·5 | Myh1 | Myosin, heavy polypeptide 1 | 4·0 | Erbb3 | v-erb-b2 homolog 3 |
| 4·5 | Fabp4 | Fatty acid binding protein 4, adipocyte | 3·9 | Dst | Dystonin |
| 4·5 | St8sia2 | ST8 sialyltransferase 2 | 3·9 | Xist | Inactive X specific transcripts |
| 4·5 | Tgfb1 | Transforming growth factor, β 1 | 3·8 | Arhgdig | Rho GDP dissociation inhibitor γ |
| 4·3 | Pscdbp | Pleckstrin homology binding protein | 3·7 | Edn1 | Endothelin 1 |
| 4·2 | Igf1 | Insulin-like growth factor 1 | 3·7 | 6330403K07Rik | RIKEN cDNA 6330403K07 gene |
| 4·1 | Scara5 | Scavenger receptor class A, member 5 | 3·7 | Jun | Jun oncogene |
| 4·1 | Sept6 | Septin 6 | 3·6 | Pla2g5 | Phospholipase A2, group V |
| 4·0 | D17H6S56E-5 | Chr 17, human D6S56E 5 | 3·6 | Apbb2 | Amyloid β precursor protein-binding B2 |
| 4·0 | Pappa | Pregnancy-associated plasma protein A | 3·6 | H19 | H19 fetal liver mRNA |
| 4·0 | Klk1 | Kallikrein 1 | 3·6 | Hspb1 | Heat shock protein 1 |
| 3·9 | Dmkn | Dermokine | 3·6 | Fcgr2b | Fc receptor, IgG, low affinity IIb |
| 3·8 | Tpd52l1 | Tumour protein D52-like 1 | 3·5 | Transcribed locusc | |
| 3·8 | Inha | Inhibin α | 3·5 | Adi1 | Acireductone dioxygenase 1 |
| 3·8 | Slc25a5 | Solute carrier family 25, member 5 | 3·4 | H2-T23 | Histocompatibility 2, T region locus 23 |
| 3·7 | Car3 | Carbonic anhydrase 3 | 3·4 | Scin | Scinderin |
| 3·7 | Pde4b | Phosphodiesterase 4B, cAMP specific | 3·3 | Rnasel | Ribonuclease L |
a
If a transcript is represented more than once on the array only the highest fold change is shown in this table. Supplementary Table 2 shows the complete significant dataset.
b
Net affy number 1454967_at.
c
Net affy number 1436092_at.