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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Nat Chem Biol. 2009 Feb 22;5(4):220–226. doi: 10.1038/nchembio.150

Figure 3.

Figure 3

The virtual screening hit Ned-19 antagonizes Ca2+ signaling in intact cells. (a) Ned-19 (100 μM in the bath) inhibits NAADP-induced Ca2+ release in an intact sea urchin egg. Ca2+ was monitored with the Ca2+-reporting dye Oregon Green 488-BAPTA Dextran (10 μM, microinjected). NAADP was pressure microinjected (100 μM in the pipette) so that each injection delivered approximately 1 μM. (b) Ned-19 inhibits NAADP-induced Ca2+ increases in mouse pancreatic beta cells. Ca2+-dependent current traces are from beta cells that were voltage-clamped with a patch pipette containing 100 nM NAADP in the presence or absence of 100 μM Ned-19 in the bath.