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The virtual screening hit Ned-19 is fluorescent and labels receptors in intact cells. (a) Excitation (368 nm) and emission (425 nm) spectra of Ned-19. Ned-19 was in dimethylsulfoxide at 4 mM. (b) Ned-19 but not Lysotracker Red labeling of pancreatic beta cells is resistant to the V-type proton pump inhibitor bafilomycin A1. Lyostracker red but not Ned-19 labeling is resistant to pre-treatment with NAADP-acetyoxymethyl ester (NAADP-AM). Scale bars, 5 μm.
