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Time course for conversion of 17,20(OH)2D2 to 17,20,24(OH)3D2 in cyclodextrin. 17,20(OH)2D2 (50 μM) dissolved in 0.45% cyclodextrin was incubated with 1.0 μM P450scc. Samples were taken at the times indicated, and product was measured by HPLC.
