Skip to main content
. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: J Mol Biol. 2009 Feb 20;387(3):744–758. doi: 10.1016/j.jmb.2009.02.020

Figure 7. Kinetics of the dissociation of the R1R2:WT 12-RSS as a function of competitor DNA.

Figure 7

(A) Time course of the dissociation of reconstituted R1R2 complex bound to radiolabeled WT 12-RSS upon addition of a 120-fold molar excess of unlabeled WT 12-RSS competitor. At the indicated times, aliquots of the sample were loaded onto a 6% nondenaturing polyacrylamide gel. The difference in migration position of equivalent bands between lanes arises from loading of the sample aliquots sequentially on a running gel. (B) Kinetics of the dissociation of the reconstituted R1R2:WT 12-RSS complex. Representative plot of fraction DNA bound versus time was fit using data from Panel A as described in Figure 4.