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. Author manuscript; available in PMC: 2010 Apr 3.

Published in final edited form as: J Mol Biol. 2009 Feb 20;387(3):744–758. doi: 10.1016/j.jmb.2009.02.020

Table 1.

Temperature dependence of MCR1 interaction with DNA substrates.

	WT 12-RSS		MHMN
Temp (K)	K_dapp (nM)	ΔG (kcal/mol)^a	K_dapp (nM)	ΔG (kcal/mol)^a

278	61 ± 5	−9.14	38 ± 9	−9.40
283	32 ± 8	−9.67	25 ± 6	−9.81
288	48 ± 9	−9.61	68 ± 9	−9.41
293	49 ± 10	−9.76	88 ± 10	−9.42
298	27 ± 6	−10.28	28 ± 8	−10.26
303	80 ± 15	−9.80	107 ± 11	−9.63
310	123 ± 23	−9.77	340 ± 57	−9.14

^a

ΔG was calculated using equation 2. Propagated errors on ΔG are all within ± 0.25 kcal/mol.