Figure 2.

Epithelial aggregate separation and isolation (EASI) of rat bladder urothelium. Hematoxylin staining of scraped bladders. (a) and (b) saline-treated bladders, (c) and (d) SP-treated bladders. EASI only removes urothelium leaving intact lamina propria. Calibration bar—(a), (c) = 400 μm; (b), (d) = 100 μm. (e) Vimentin Western blot of total urothelial cell lysates. Total protein from isolated urothelial cells was separated on 4–12% bis tris acrylamide gels and transferred to PVDF. Lanes 1–6 saline-treated animals; lanes 7–12 SP-treated animals, lane S—10 μg of scrapped bladder protein (positive control). Vimentin Western blot was stripped and reprobed with GAPDH which served as a loading control.