Figure 4.

(a) MIF and CD74 mRNA expressions in isolated urothelial cells. Total RNA was isolated from urothelial cells and CD74 or MIF-specific primers used to amplify mRNA by endpoint PCR. A CD74 and MIF mRNA is increased in isolated urothelial cells after SP treatment. Data are representative of 5 salines and 5 SP-treated animals. 18S rRNA was used as a control for the amount of total RNA added to each reaction tube. (b) Relative expression of CD74 and MIF in isolated urothelial cells. Data are expressed as fold change calculated by determining mean gene expression ratio (net intensity of gene-specific band divided by net intensity of 18S rRNA band) normalized to saline controls. (***P < .001, n = 5).