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Immunohistochemistry of bladder neurons from CYP-treated and control rats. Fluorogold (FG; left column) was used to retrogradely label LS and TL bladder neurons (arrows), which were counterstained with P2X3 antibody (middle column). Merging the left and middle columns confirmed that bladder sensory neurons were immunoreactive for P2X3 (right column; arrows indicate colocalization).
