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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: J Am Coll Cardiol. 2008 Oct 28;52(18):1468–1481. doi: 10.1016/j.jacc.2008.07.047

Figure 4. Function of CHGA 3’-UTR variants.

Figure 4

Panel A. Sequence alignment of the CHGA 3’-UTR across different species. 3’-UTR sequences are aligned across 7 species, to illustrate conservation at positions of three naturally occurring human polymorphisms: C+87T (C11825T), C+96T (C11834T), and C+274T (C12012T).

Panel B. Human CHGA naturally occurring 3’-UTR variants: Effects on transfected reporter gene expression in chromaffin cells. The 3’-UTR variants tested were: C+87T (C11825T), C+96T (C11834T), and C+274T (C12012T). Results of luciferase activity measurements 24 hours after transfection of the 4 versions of the 407-bp CHGA 3’-UTR (wild type and 3 variants) into chromaffin cells. Results are expressed as the ratio of firefly luciferase/Renilla luciferase (encoded by the transfection efficiency plasmid pRL-TK). Each experiment was performed in triplicate, and such experiments were repeated at least twice. Wild-type contains the major (C) allele at all 3 positions. Variant constructs contain the minor (T) allele at the indicated position, but the major (C) at the two other positions.