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. 2009 Mar 31;4(3):e4943. doi: 10.1371/journal.pone.0004943

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Ogonuki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a) Vasa–Venus transgenic strain. All markers (n = 74) were homozygous for B6 at N5 on day 188. b) Ednra^EGFP/+ knockin strain. All markers (n = 86) were homozygous for B6 at N3 on day 106 and N4 offspring were obtained on day 151. c) ENU-induced Gdf5 mutant strain. The N5 generation was obtained on day 190 and was 97.7% (172/176) homozygous for B6. There were 74, 86 and 176 polymorphic markers, which identified the alleles for the C57BL/6Cr:DBA/2Cr, C57BL/6Cr:129 and C57BL/6J:DBA/2J strains, respectively. Each generation turnover was between 42 and 45 days: the age of the donor male plus the gestation period (20 days) minus the one-day overlap between them.