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. 2009 Mar 31;4(3):e5070. doi: 10.1371/journal.pone.0005070

Figure 3. Sg4E-BP inhibits protein synthesis activity in sea urchin cell-free cap-dependent translation system.

Figure 3

Cell-free translation system from unfertilized eggs (UF) and 30 minutes post-fertilization embryos (F) were prepared as described in Materials and Methods. (A) Renilla Luciferase activity was measured after addition of capped (Cap+) or uncapped (Cap−) mRNA. Error bars represent the standard deviation (s.d.) of duplicates. (B) Sg4E-BP inhibits cap dependent translation activity and this inhibition is rescued by eIF4E. 100 ng of GST (lane 1), 100 ng of GST-Sg4E-BP alone (4E-BP, lane 2) or preincubated 5 min with 250 ng of GST-mIF4E (4E-BP+eIF4E, lane 3) were added to the fertilized cell free translation system and Luciferase activity was measured as described in Materials and Methods after addition of a capped mRNA encoding Renilla Luciferase. Error bars represent the standard deviation (s.d.) of duplicates. Significance was assessed using Fisher's F-test and Student's t-test. *P<0.005, significant difference between 4E-BP and GST, and between 4E-BP and 4E-BP+eIF4E.