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. 2009 Mar 31;4(3):e5074. doi: 10.1371/journal.pone.0005074

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Debellis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Caco-2 cells, loaded with MEQ were incubated with (thick line) or without (thin line) 500 pM VCC. Fluorescence was estimated upon sequential buffer substitution with Cl⁻- containing medium (# 1) followed by Cl⁻-free medium (# 2). Substitution of solution (#1) by solution (#2) led to an increase of MEQ fluorescence (dequenching). Subsequent substitution by solution #1 induced an influx of Cl⁻ ions, thus quenching MEQ fluorescence. Values are normalized with respect to the base line. B) Effect of 100 µM DIDS on VCC-induced chloride efflux. ΔF has been calculated as reported in the Material and Methods section. Each histogram represents an average of thirteen independent experiments with a recording of 2–3 cells in each assay. Significance, determined by Student's t test, was compared to non-treated cells (Ctrl); ***, p<0.001.