Table 1. de novo heterochromatin domains are associated with reduced gene expression at both the ura4 and spbc2f12.03 loci.
| Gene1 | Gene name | Distance from L5 (bp)2 | Relative mRNA (+L5/−L5) | N3 | p4 |
| A | alg115 | 8393 | 1.02±0.11 | 8 | 0.853 |
| B | CC330.07 | 6245 | 1.21±0.10 | 11 | 0.101 |
| C | spcc330.06 | 2723 | 0.57±0.10 | 14 | 0.002 |
| ade6 | 340 | 0.67±0.05 | 13 | 0.000 | |
| D | tDNAgly | 4535 | |||
| E | mug135 | 4943 | 0.48±0.08 | 8 | 0.001 |
| F | CC330.03 | 7819 | 0.90±0.20 | 17 | 0.628 |
| G | CC330.19 | 9984 | 0.66±0.03 | 8 | 0.008 |
| H | rhp7 | 10902 | 1.08±0.15 | 4 | 0.731 |
| 1 | mlo36 | 10609 | 0.83±0.09 | 5 | 0.172 |
| 2 | byr2 | 8193 | 0.67±0.15 | 6 | 0.284 |
| 3 | mrpl7 | 4875 | 0.81±0.15 | 6 | 0.289 |
| ade6 | 340 | 0.48±0.10 | 5 | 0.001 | |
| 4 | rpl1701 | 4028 | 0.58±0.08 | 5 | 0.007 |
| 5 | BC2f12.05 | 9177 | 0.62±0.18 | 6 | 0.087 |
| 6 | rpl802 | 11334 | 1.03±0.08 | 5 | 0.752 |
| 7 | ceg17 | 13164 | 1.08±0.11 | 7 | 0.335 |
1
From Figure 1A and 1C.
2
Distance of the translation start site from the nearest edge of L5.
3
Number of independent RNA isolations included in analysis.
4
p-value resulting from comparison between ura4::ade6+ and ura4::L5-ade6+ strains.
5
Essential for viability [82].
6
Mutation in mlo3 results in a growth defect [83].
7
Essential for viability [84].
Relative gene expression in the ura4 and spbc2f12.03 loci. Steady-state mRNA for genes in the ura4 (A–H) and spbc2f12.03 (1–7) loci were assayed via quantitative RT–PCR and are reported relative to ura4::ade6+ control strains. The distance from the transcription start site to the nearest edge of L5 is indicated for each gene.