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. 2009 Mar 31;4(3):e5076. doi: 10.1371/journal.pone.0005076

Figure 5. Increased Su(z)2 provides for activation of Myc targets, and a reduction in their H3K27 tri-methylation.

Figure 5

A) RT-PCR analysis showing expression of three Myc activation targets (indicated on the left side of the gel pictures) in embryos of 4 different genotypes (indicated above the lanes). Average band intensities (Quantity 1) of biological triplicates are plotted on the right, with relative standard deviations indicated. B) ChIP assay showing histone H3K27 tri-methylation at five Myc activation targets (indicated to the left of the gel pictures) in embryos of genotypes indicated on top. Data shown on the left are plotted in a stacked column chart; the y-axis is the density of each PCR product divided by the density of the input sample PCR product, and the values for each gene are stacked together for each of the four genotypes. These data show a representative set of biological triplicates.