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. 2009 Apr 1;4(4):e5103. doi: 10.1371/journal.pone.0005103

Figure 3. TFIID present in nuclear extracts contributes in X mRNA transcription, but TAF11 is dispensable.

Figure 3

(A) In vitro transcription assays of immmunodepleted nuclear extracts (NEs). To examine importance of TFIID for X gene transcription, TFIID was depleted from NEs using antibodies raised against different TFIID subunits and the depleted NEs were tested for X gene transcription activity using the HBV enhancer-X promoter construct. SS1 and SS2: primer extension products showing transcription from Start site 1 and Start site 2, respectively. The asterisk shows a primer extension product that was not consistently observed. (B) Western blot analyses of the immunodepleted NEs. Depleted NEs were examined for the levels of TAF1, TAF4, TAF6, TAF11, and TBP.