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Specific anti-tumor cytotoxicity of 2nd generation designer T cells. Untransduced, 1st and 2nd generation designer T cells were incubated at specified ratios of designer CTL effectors to MIPCEA targets (E:T) for 4 hrs in a chromium-release assay. CEA(−) MIP101 cells in parallel assays were <5% lysed at the highest E:T ratios (not shown).
