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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2008 Nov 6;40(2):187–198. doi: 10.1016/j.mcn.2008.10.006

Fig. 9.

Fig. 9

Down-regulation of ash1 and ngn2 expression in retinas infected by RCAS-ngn3. (A, B) Expression of ash1 in normal E7.5 retina at the periphery (A) and the central region (B). (C, D) Serial sections of an E7.5 eye infected with RCAS-ngn3 and stained for viral protein p27 to identify regions infected by the virus (C) or stained for ash1 mRNA by in situ hybridization (D). Arrows point to corresponding regions lacking viral infection (C) and retaining ash1 expression (D). (E, F) Double-labeling for viral protein p27 (E) and ash1 expression (F) of E5 retina partially infected with RCAS-ngn3. Yellow lines indicate infected regions. (G, H) The spatial expression patterns of ngn2 in the control infected with RCASGFP (G) and experimental (H) retina. The cellular layer with diminished gene expression is marked by a red bar. Scale bars: 100 µm.