FIG. 1.

Transfection of FAK siRNA in osteoblasts. (A) Western blot analysis of FAK and Pyk2 expression and activation (phosphorylation of Y397, Y402) in osteoblasts treated with lipofectamine only (Lipo Only), 50 nM scrambled control siRNA (Scramble), or 50 nM FAK siRNA smart pool (FAK siRNA) compared with untreated osteoblasts (Unt). Vinculin was analyzed as a loading control. (B and C) Quantification of FAK expression and FAK activation as measured by phosphorylation of Y397 expressed as raw densitometry units (^a p < 0.05). (D and E) Quantification of Pyk2 expression and Pyk2 activation as measured by phosphorylation of Y402 expressed as raw densitometry units. Error bars represent SE.