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. 2008 Nov 3;24(3):411–424. doi: 10.1359/JBMR.081102

FIG. 2.

FIG. 2

Analysis of osteoblasts infected with FAK-related nonkinase (FRNK) and FAK+/+ and FAK−/− osteoblasts. (A) Western blot analysis of FAK activation (phosphorylation at Y397), FRNK expression, Pyk2 expression, and activation (phosphorylation at Y402) in osteoblasts infected with GFP only control (GFP) or FRNK-GFP (FRNK) compared with uninfected osteoblasts. Arrow indicates GFP-FRNK; asterisk indicates endogenous FRNK. Vinculin was analyzed as a loading control. (B) Quantification of FRNK expression and FAK activation after infection as compared with uninfected osteoblasts expressed in raw densitometry units. (C) Quantification of Pyk2 expression and activation after infection compared with uninfected osteoblasts expressed in raw densitometry units. (D) Western blot analysis of control osteoblasts clone (1C, 1E11) and FAK−/− osteoblast clones (1D8, 4F1) shows no FAK expression wildtype levels of Pyk2 expression in the FAK−/− clones. FAK−/− clones exhibit similar levels of activated Pyk2 as measured by phosphorylation at Y402 compared with control clones. (E) Quantification of Pyk2 expression and activation in the control and FAK−/− osteoblasts clones expressed in raw densitometry units (a p < 0.05). Error bars represent SE.