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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2008 Sep 23;45(12):1682–1694. doi: 10.1016/j.freeradbiomed.2008.09.009

Fig. 5.

Fig. 5

UV-visible absorbance spectra of human partially purified full-length VPO1. A. Spectra of expressed, partially purified VPO1 are shown, including oxidized VPO1, oxidized VPO1 in pyridine (pyr), reduced VPO1 in pyridine, and oxidized VPO1 in 88% formic acid. B. Reduced spectra of pyridine hemochrome. Reduced spectra of pyridine hemochrome were obtained by addition of pyridine to 2.4 M and a few crystals of sodium dithionite. The reduced spectrum showed a split Soret band with features at 393 and 413 nm. The presence of the symmetrical Soret peak of VPO1 in above conditions provides evidence for a native environment for the heme. C. Spectra from 450 nm to 700 nm of B.