Table 2. Relative activity comparing with LPO.
Relative activity is expressed as percentage of LPO activity. Luminol oxidation was carried out in HBSS solution with 1 mM luminol and 100 µM H2O2.RLU was recorded using a FluoStar™ luminometer. Data are the average of three independent experiments.
Component | Luminol assay (Relative %) |
---|---|
LPO | 100 |
VPO1 | 4.0 ± 0.2 |
Hematin | 0.5 ± 0.1 |
Myoglobin | 0.6 ± 0.1 |
Cytochrome c | 1.0 ± 0.1 |