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. Author manuscript; available in PMC: 2009 Mar 23.
Published in final edited form as: J Biol Chem. 2007 Feb 6;282(14):10172–10179. doi: 10.1074/jbc.M611210200

FIGURE 5. LPA attenuated IL-13-induced STAT6 phosphorylation in HBEpCS.

FIGURE 5

A, HBEpCs grown to ∼70–80% confluence were treated with LPA (1 μM) for 6 h. Cells were challenged with IL-13 (5, 10, and 20 ng/ml) and IL-4 (10 ng/ml) for 15 min. Cell lysates were subjected to 10% SDS-PAGE and analyzed to specific antibodies to phospho-STAT6 or total STAT6. This is a quantitative analysis from three independent experiments (mean ± S.D.). *, p < 0.05 versus IL-13 (10 ng/ml)-treated cells; **, p < 0.05 versus IL-13 (20 ng/ml)-treated cells. B, HBEpCs grown to ∼40–50% confluence were transfected with scrambled (Scramb) or IL-13Rα2 siRNAs for 72 h. Cells were pretreated with LPA (1 μM) for 6 h and then challenged with IL-13 (10 ng/ml) for 15 min. Cell lysates were subjected to 10% SDS-PAGE and analyzed to specific antibodies to phospho-STAT6 or total STAT6. This is a quantitative analysis from three independent experiments (mean ± S.D.). *, p < 0.05 versus IL-13 (10 ng/ml)-treated cells. C, cells were treated as described in B. Media were collected, subjected to 10% SDS-PAGE, and analyzed with specific antibody to IL-13Rα2. This is a quantitative analysis from three independent experiments (mean ± S.D.). *, p < 0.05 versus scrambled siRNA transfected cells; **, p < 0.05 versus scrambled siRNA + LPA treatment.