TABLE 1.
Primers for RT-PCR | |
IL-13Rα1 | Forward, 5′-GGAGAATACATCTTGTTTCATGG-3′ |
Reverse, 5′-GCGCTTCTTACCTATACTCATTTCTTGG-3′ | |
IL-13Rα2 | Forward, 5′-AATGGCTTTCGTTTGCTTGG-3′ |
Reverse, 5′-ACGCAATCCATATCCTGAAC-3′ | |
IL-4Rα | Forward, 5′-GACCTGGAGCAACCCGTATC-3′ |
Reverse, 5′-CATAGCACAACAGGCAGACG-3′ | |
GAPDHa | Forward, 5′-GGAGCCAAAAGGGTCATCATCT-3′ |
Reverse, 5′-AGTGGGTGTCGCTGTTGAAGT-3′ | |
Primers for real-time RT-PCR | |
IL-13Rα2 | Forward, 5′-ACTGGTATGAGGGCTTGGAT-3′ |
Reverse, 5′-TCTGATGCCTCCAAATAGGG-3′ | |
SOCS-1 | Forward, 5′-TTGGAGGGAGCGGATGGGTGTAG-3′ |
Reverse, 5′-AGAGGTAGGAGGTGCGAGTTCAGGTC-3′ | |
Eotaxin-1 | Forward, 5′-AACCACCTGCTGCTTTAACC-3′ |
Reverse, 5′-CACAGCTTTCTGGGGACATT-3′ | |
18S | Forward, 5′-GTAACCCGTTGAACCCCATT-3′ |
Reverse, 5′-CCATCCAATCGGTAGTAGCG-3′ |
Glyceraldehyde-3-phosphate dehydrogenase.