Figure 3.

Schematic overview of discovery-oriented pipeline. The 384 targets were divided into four sets of 96 targets each (SC-1, SC-2, SC-3 and SC-4). Each tier shows the number of targets that were successfully passed through the specified stage of the pipeline (cloning, expression, solubilization and size exclusion) with the percent success rate relative to the previous tier. SC-1 targets which expressed and solubilized in DDM were then scaled-up to size exclusion to determine if the protein was present within the included volume of the column. 31 proteins, of 61 attempted, were shown to be present within the included volume at a level of 50% or greater. Of these, 23 proteins were fully included under the conditions tested. Five of these targets were pushed into the intensive production phase and determined to be readily purifiable and stable (“HS”) (Figure 5).