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. Author manuscript; available in PMC: 2010 Jan 23.
Published in final edited form as: J Mol Biol. 2008 Nov 24;385(3):820–830. doi: 10.1016/j.jmb.2008.11.021

Figure 5.

Figure 5

Representative set of eukaryotic integral membrane proteins identified within this screen. Each target is fully cleaved and injected on a Superdex 200 size exclusion column (approximately 24 ml bed volume flowing at 0.33 ml/minute) on day one (red) and day fourteen (blue) following storage at 4°C. Q14542 was reinjected on day eight. Each target has a symmetrical, unnormalized, peak with the ordinate being absorbance at 280 nm wavelength (in milli-absorbance units) and abscissa being column time (in minutes). In addition, a Coomassie stained SDS-PAGE gel is shown for each sample to demonstrate purity. In each case the primary band corresponds to the correct molecular weight for the specified target. The Precision Plus Protein Standard (BIO-RAD) standard is included with the corresponding molecular weights (top to bottom in kD): 250, 150, 100, 75, 50, 37, 25, 20, 15 and 10. A UniProt ID identifies each target.