Table 2.
Antibodies used for immunohistochemistry
| Antigen | Sp. | Dilution | Source | Catalog # |
|---|---|---|---|---|
| βIII Tubulin | Ms | 1:500 | Covance, Berkeley, CA | MMS-435P |
| β-Actin | Rb | 1:1000 | AbCam, Cambridge, MA | ab8227 |
| BDNF | Rb | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA | sc-546 |
| β-Gal | Ms | 1:500 | Promega, Madison, WI | Z3781 |
| BrdU | Rt | 1:100 | AbCam, Cambridge, MA | ab6326 |
| Calbindin | Ms | 1:1000 | Swant, Bellinzona, Switzerland | 300 |
| GFAP | Ms | 1:500 | Chemicon, Temecula, CA | MAB3402 |
| GFP | Ch | 1:500 | Aves Labs, Tigard, OR | GFP-1020 |
| HuC/D | Ms | 1:10 | Molecular Probes, Eugene, OR | MAB16A11 |
| Mash1 | Ms | 1:150 | BD Pharmingen, San Jose, CA | 556604 |
| p75 (extra-c.) | Rb | 1:2000 | Louis Reichardt (Weskamp and Reichardt, 1991) | |
| p75 (intra-c.) | Rb | 1:1000 | Promega, Madison, WI | G3231 |
| p75 (extra-c.) | Ms | 1:100 | Chemicon, Temecula, CA | MAB365 |
| p75 (extra-c.) | Rb | 1:500 | Moses Chao (9651) (Huber and Chao, 1995) | |
| p75 (intra-c.) | Rb | 1:500 | Moses Chao (9992) | |
| pH3 | Rb | 1:200 | Upstate, Lake Placid, NY | 06–570 |
| PSA-NCAM | Ms | 1:500 | AbCys, Paris, France | AbC0019 |
| pTrkB | Rb | 1:200/1:1000 | Moses Chao (Bath et al., 2008) | |
| TrkB | Rb | 1:200/1:750 | Upstate, Lake Placid, NY | 07–225 |
| TrkB-TR | Rb | 1:200 | Santa Cruz Biotechnology, Santa Cruz, CA | sc-119 |
| TrkB | Rb | 1:200 | Chemicon, Temecula, CA | AB9872 |
| TrkB | Rb | 1:200/1:750 | Chemicon, Temecula, CA | AB5372 |
| TH | Rb | 1:500 | PelFreez, Rogers, AR | P40101 |
Whenever different dilutions were used for immunohistochemistry and Western blot, both dilutions are presented in that order. Sp., species in which antibody was raised; Ms, mouse; Rt, rat; Rb, rabbit; Ch, chicken; extra-c, extracellular; intra-c, intracellular.