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. Author manuscript; available in PMC: 2009 Mar 23.
Published in final edited form as: J Neurochem. 2008 Jan 12;105(3):956–970. doi: 10.1111/j.1471-4159.2007.05199.x

Figure 1. Factors released by LPS-stimulated glial cells (LCM) inhibit OL development which is attenuated by NAC.

Figure 1

OL-progenitors generated from rat mixed glial cultures were cultured in conditioned media generated from mixed glial cultures treated with LPS (LCM), NAC plus LPS (NLCM), NAC alone (NCM) and/or untreated control (CCM) as described under ‘Materials and Methods’. Plot depicts percentage of NG2+/BrdU+ cells/10-fields/slide, 24 h post-culturing (A). Plot depicts comparison of total NG2 with NG2+/BrdU- cell population/5-fields/slide in similarly treated cells (B). Representative field of slides demonstrates NG2+ and BrdU+ cells counter stained with nuclei stain (Hoechst) at 24 h post-culturing (C). Plots depict percentage change in count of O4+ (late OL progenitors) (D) and MBP+ (differentiating OLs) (E) in cultured OL progenitors for 72 h and 144 h, respectively. Plot data are expressed as Mean ± SD of 3 independent experiments conducted in triplicate. Statistical significance is indicated as **p< 0.01 and ***p<0.001 versus LCM, and $p<0.05 versus CCM. Asterisks **<0.01 and ***p< 0.001 and NS (non-significant) (B).