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. Author manuscript; available in PMC: 2009 Mar 23.
Published in final edited form as: J Neurochem. 2008 Jan 12;105(3):956–970. doi: 10.1111/j.1471-4159.2007.05199.x

Figure 5. LCM consists of proinflammatory cytokines (TNF-α and IL-1β) involved in the ROS generation and peroxisomal dysfunction in developing OLs.

Figure 5

Rat developing OLs and astrocytes were treated separately with a cocktail of pro-inflammatory cytokines (Cyt-Mix: TNF-α and IL-1β; 10 ng/ml each) in the presence/absence of NAC (10 mM) or WY (20 μM) for 48 h followed by determination of VLCFAs accumulation and ROS generation. Plots depict VLCFAs (C26:0/22:0 ratio) in developing OLs (A) and astrocytes (B). Plots depict ROS generation in similarly treated developing OLs (C) and astrocytes (D). Further, rat developing OLs and astrocytes were exposed to LCM for 48 h in the presence/absence of anti-IL1R1 and –TNFR1 MAbs in combination or individually. Plots depict ROS generation in treated developing OLs (E) and astrocytes (F) for 48 h. Plot data are presented as Mean ± SD of three identical experiments. Statistical significance is indicated as **p<0.01and NS (non-significant) versus Cyt-Mix (A-D) and *p< 0.05 and **p<0.01 versus LCM (E and F).