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. Author manuscript; available in PMC: 2009 Mar 23.
Published in final edited form as: J Neurochem. 2008 Jan 12;105(3):956–970. doi: 10.1111/j.1471-4159.2007.05199.x

Figure 6. Similar to LCM, diamide treatment (deplete GSH) reduces peroxisomes and inhibits the differentiation of developing OLs.

Figure 6

Rat primary developing OLs were treated with diamide (DIAM; 0.1–2.0 mM). Plots depict transcript levels for DHAP-AT (A), PMP70 (B), PPAR-α (C), and MBP (D) in the developing OLs 48 h post-treatment. Plot depicts LDH release in the diamide-treated developing OLs for 48 h (E). Although real-time PCR amplified products for PMP70 and MBP genes were detected in 2% agarose gel after the completion of the run, real-time PCR analysis showed a dose-dependent decrease in PMP70 and MBP transcripts in developing OLs by diamide (F). Data in plots are presented as Mean ± SD of three identical experiments. Statistical significance is indicated as *p< 0.05, **p<0.01 and ***<0.001 versus control (untreated).