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. Author manuscript; available in PMC: 2009 Mar 23.
Published in final edited form as: J Neurochem. 2008 Jan 12;105(3):956–970. doi: 10.1111/j.1471-4159.2007.05199.x

Figure 7. Knock out and antisense RNA based transfection studies established that NAC modulates PPAR-α activity in LCM exposed developing OLs.

Figure 7

To confirm the modulation of PPAR-α activity by NAC in LCM exposed developing OLs, we used PPAR-α (-/-) and wild-type developing OLs and treated similarly with LCM, NAC and WY14643 (WY) as described under methods. Plot depicts the percentage of MBP+ OLs generated from wild-type/PPAR-α (-/-) mice 48 h post-treatment analyzed by FACS (A). Plot depicts relative luciferase activity in B12 oligodendroglial cells co-transfected with pTK-PPREx3-Luc and PPAR-α siRNAs or scramble and treated with NAC (10 mM) or WY (50 μM) prior to incubation with Cyt-Mix as described under Methods (B). Plot data are presented as Mean ± SD of three identical experiments. Statistical significance is indicated as *p<0.05, **p<0.01 and NS (non-significant) versus LCM (wild-type and scramble SiRNA).