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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cell Metab. 2009 Jan 15;9(2):152–164. doi: 10.1016/j.cmet.2008.12.012

Figure 2. Hif-2α modulates iron-regulated genes in the small intestine.

Figure 2

(A) Western blot analysis duodenal epithelial cells in wild-type C57BL/6 mice receiving a low-iron diet for 2-weeks. Expression was normalized to HNF4a protein expression. Nuclear lysate from HCT116 cells treated with 1% O2 for 24-hours were used as a positive control (+ Ctrl) (B) Global gene expression profiling was assessed in duodenal RNAs isolated from VhlF/F and VhlΔIE mice or wild-type mice receiving a low-iron diet for 2-weeks. (C) qPCR analysis of selected genes in duodenal epithelial cells from VhlF/F and VhlΔIE mice or wild-type C57BL/6 mice receiving a low-iron diet for 2-weeks. Expression was normalized to β-actin and each bar represents the mean value ± S.D. (*)= P<.05.