Fig. 3.

Glucose removal immediately diminishes amiodarone induced Ca²⁺ burst and toxicity. Exponentially growing yeast cultures were spun down and resuspended in fresh SC medium with (+Glc) or without (−Glc) 2% glucose, (a) Aequorin-coelenterazine luminescence was measured after injection of 80 µM amiodarone (arrow). Data are representative of four independent experiments, (b) Viability of the cultures was assessed on YPD agar immediately following luminescence measurements in (a), as described in Fig. 2a. One of two duplicate plates from this experiment is shown. Data are representative of four independent experiments.