. Author manuscript; available in PMC: 2009 Mar 23.

Published in final edited form as: J Gene Med. 2007 Aug;9(8):668–678. doi: 10.1002/jgm.1058

Table 1.

Protein coatings in substrate-mediated DNA delivery: rank order of proteins for their influence on multiple aspects of the gene transfer process

Surface delivery	Bolus delivery	Surface-immobilized	Cell-associated	Internalized
Fibronectin (100%)	Collagen I (100%)	Fibronectin (100%)	Fibronectin (100%)	Fibronectin (100%);
	No coating (99%)	FBS (97%)	BSA (89%)	Collagen I (99%)
	Laminin (95%)	BSA (96%)	Collagen I (85%)
	BSA (33%)	Collagen I (85%)
	FBS (33%)
	Fibronectin (30%)
Collagen I (23%)		Laminin (11%)	FBS (48%)	FBS (60%)
BSA (15%)
FBS (6%)
Laminin (0.5%)			Laminin (11%)	BSA (43%)
No coating (0%)				Laminin (2%)

Percentages of maximum are indicated in parantheses. Differences in DNA levels between protein coatings listed in the same cell are not statistically significant.