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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cancer Res. 2009 Jan 27;69(3):1212–1220. doi: 10.1158/0008-5472.CAN-08-1166

Figure 1. Expression of Vstat120 enhances survival of rats implanted with U87MGD glioma cells in the brain.

Figure 1

A. Western blot analysis of cell lysates from U87MG parental cells, and U87MG-derived clones stably transfected with Vstat120 cDNA (U14 and U18). Note the expression of Vstat120 in the whole cell extract (WCE) and conditioned medium (CM) of the stably transfected clones. B. The in vitro proliferation rates of U87MG cells, and clones U14 and U18 was determined by the crystal violet assay. Note expression of Vstat120 did not alter the proliferation rate of the clones versus U87MG cells. C. CM from control U87MGD or Vstat120 expressing U14 cells were tested for their ability to inhibit the migration of HDMECs in a Transwell migration assay. The number of cells that migrated to the bottom of the chamber after 8 hrs was quantified as described in materials and methods. Random migration in response to medium alone was subtracted from the values. Expression levels of Vstat120 and thrombospondin-1 (TSP1) in the CM were assessed by Western blot. D. Intracranial tumorigenicity assay for U87MG and Vstat120 expressing clones, U14 and U18. 1 × 106 cells were implanted stereotactically in the brain of athymic nude rats. Survival curves of rats implanted with cells expressing Vstat120 showed a significant improvement in their survival compared to the control parental U87MG cells (p<0.05).