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Inserting the H-NS linker sequence into Ler reduced, but did not eliminate, the ability of Ler to increase LEE5 activity. β-Galactosidase activities were derived from LEE5–lacZ fusions in the presence of Ler proteins containing either the H-NS linker sequence ELLNSLAAVKS (pFL08), 11 alanines (pFL10) or six alanines (pFL09) inserted at amino acid position 67. β- Galactosidase assays were performed as described for Fig. 2.
