Fig. 5.

In vitro SNC80 potentiates amphetamine-induced dopamine efflux when administered directly to striatal preparations or P2 striatal synaptosomes from naïve rats. Striatal slices were perfused with: a. either vehicle or 10 µM SNC80 for 15, 30 or 60 min prior to amphetamine or b. 1 µM, 10 µM, or 100 µM SNC80 for 30 min before amphetamine. c. P2 synaptosomal fractions were perfused with 1µM, 3 µM, or 10 µM SNC80 for 30 min before amphetamine. All treatments received 100 µM amphetamine sulfate for 5 min and SNC80 administration was discontinued during the addition of amphetamine and subsequent perfusates. Samples were collected at 5 min intervals for an additional 40 min. Dopamine content of the fractions was measured by HPLC-electrochemical detection as described in the Methods and plotted AUC (± S.E.M.) as measured from the release curves (fractions 4–15) of each treatment group (n = 4 in duplicate). Statistical significance was determined in a. by two-way ANOVA with Bonferroni multiple comparison analysis and in b. by one-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle.