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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Biochem Pharmacol. 2008 Dec 31;77(7):1151–1160. doi: 10.1016/j.bcp.2008.12.015

Fig. 5.

Fig. 5

Direct inhibition of VEGF secretion by TRAMP mouse dorsolateral prostates cultured in the presence of 6-MCDF. Pieces from tumor-free 140-day-old TRAMP dorsolateral prostates were minced and incubated for three days in organ culture media containing 0, 100 or 1000 nM 6-MCDF. For each mouse, pieces taken from the same prostate were cultured in duplicate for each concentration of 6-MCDF. Prostate histology was determined for two pieces not selected for organ culture, and confirmed after 3 day organ culture on individual prostate pieces. VEGF concentrations relative to weight of each cultured prostate piece were determined in the culture media by ELISA after the final 24 hr period. For each mouse prostate, VEGF levels were expressed as a percentage of the mean for the pieces taken from the same prostate cultured without 6-MCDF. Values are the mean ± SEM for 0 (n = 15), 100 (n = 12) or 1000 nM (n = 15) 6-MCDF in TRAMP mice. * indicates a significant difference compared to vehicle group (p < 0.05).